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Figure Lengend Snippet: SYT-1 protein levels are unchanged, but SNAP-25 protein expression was decreased in the cortex of NL3 R451C mice. SYT-1 and SNAP-25 protein expression in cortical ( A , G ), striatal ( C , I ) and cerebellar ( E , K ) lysates from NL3 R451C and WT mice were analysed via Western blot. Densitometric analysis was performed to demonstrate quantitative expression of SYT-1 ( B ,D, F ) and SNAP-25 ( H , J , L ) relative to β actin expression. Genotype differences were analysed using an unpaired, two-tailed Student’s t-test (n = 6 mice in each group); **p < 0.01. Data represented as mean ± SEM.

Article Snippet: Rabbit polyclonal anti-SYT-1 (48 kDa) , Alomone Labs #ANR-003 , 1:500.

Techniques: Expressing, Western Blot, Two Tailed Test

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Figure Lengend Snippet: Antibodies used in Western blot analysis.

Article Snippet: Rabbit polyclonal anti-SYT-1 (48 kDa) , Alomone Labs #ANR-003 , 1:500.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Downregulation of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) in a co-culture system with human stimulated X-linked CGD neutrophils

doi: 10.1371/journal.pone.0230665

Figure Lengend Snippet: (A) Total protein expression of NFκb p65 and phosphorylated at Ser536 in HUVECs in the neutrophil-containing transwell system compared with GAPDH expression. (B) Quantification of total NFκb p65. (C) Quantification of phosphorylation at Ser536 position compared with total NFκb p65.

Article Snippet: For immunodetection, membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 (TBST) and incubated in TBST with eNOS and phosphorylated-eNOS(Ser1177) antibodies (Cell life signaling, Danvers, MA, USA) (1:100 dilution), anti-EDN1 antibody (Sigma-Aldrich, St. Louis, MO, USA) (1:500), anti- NFκB (p65) antibody, and anti-phosphorylated NFκB (p65) at Ser536 position (St. John’s Laboratory, London, UK) (1:500), and anti-GAPDH antibody (Sigma-Aldrich, St. Louis, MO, USA) (1:1000) with chemiluminescent detection.

Techniques: Expressing, Phospho-proteomics